Immunocytochemical Reactions in the Brittle-Star: Ophiocomina Nigra after Immunization

Abstract

Immunizations of Ophiocomina nigra were performed with either Bovine Serum Albumine (BSA) or Horse-Radish
Peroxydase (HRP) in aquarium containing running sea water. An immunocytochemical test was done in presence of
HRP, diaminobenzidin, H2O2 for both animals: Control ones, BSA ones and HRP ones. Positive reactions only occur in
injected animals to HRP. So the brittle star O.nigra as the sea stars: Asterina gibbosa discriminates HRP from BSA: it is
an antibody-like reaction. Plasmolymphocytic cells were evoked.