Investigation of Phytochemical Profile and Combine Synergistic Anti-Diarrheal Potential of Peperomia Pellucida Aerial Part and Cyprus Esculentus Root Extracts

Abstract

Peperomia pellucida and Cyperus esculentuslocally found plant used for anti-diarrhoeal activity using standard animal model. The aerial parts of Peperomia pellucida and Cyperus esculentus were collected from Trikaripur forest area, kasaragod district of Kerala, India, dried and extracted with ethanol (70%). Wistar albino rats were used for studies. Castor oil was used to induce diarrhoea and 200, 400 mg/kg of ethanol extract of Peperomia pellucida and Cyperus esculentus dual combination was used to test antidiarrhoeal activity. Results were calculated by student “t” test, to assess statistical significance. The extract showed dose dependant inhibition of frequency of defecation as well as reduction in number of wet feces. The percentage inhibitions of faecal with 200 and 400 mg/kg doses of ethanol extract were 69.15 and 76.21 respectively. The plant extracts contains tannins and flavonoids, which could have contribution to the anti-diarrhoeal activity. Peperomia pellucida and Cyperus esculentus combine hydro-ethanolic extractpossesses significant antidiarrhoeal activity compared with loperamide.