In vitro Evaluation of Antioxidant Activity of Picea smithiana Growing in Bhaderwah Region of Jammu and Kashmir

Abstract

Picea smithiana is one of the plants belongs to family pinaceae that has not been explored scientifically from
pharmaceutical point of view. The present study targeted to explore the antioxidant potential of Picea smithiana growing
in Bhadewah region (Shoj dhar) at the altitude of 11000 feats of Jammu and Kashmir, India. Three types of extracts were
prepared from leaf and Bark part of the plant viz. chloroform, methanol and aqueous and analyzed for their total phenols
and flavonoid content. Antioxidant potential of the extracts were analyzed by four different method viz., DPPH radical
scavenging method, Fe2+ ion chelating method, FRAP assay and Potassium ferric cyanide reduction method.
The results of the study determined that methanolic extract of leaf part contained good content of phenolic compound
(70.4 ± 2.1mg GAE/g dw) which contributed as good antiradical (IC50 value 228 ± 3.2µg/ml), chelation activity (55 ± 1.5%
at 500µg), FRAP (494 ± 5.2μmol Fe (II)/g) and Potassium ferric cyanide reduction activity (EC50 value of 978µg/ml). A
correlation between the antioxidant activity (FRAP) and the phenolic content of Picea smithiana extracts has also been
drawn and found significant (R²=0.965). In comparison, bark extracts possess less poly phenols that confer poor
antioxidant potential.